4.3. Cell Viability Assay

A CCK8 kit (CK04) was employed to measure the cell viability. Briefly, the cells were exposed to indicated concentrations of t-BHP and/or indicated concentrations of DPDS for 24 h. After that, 10 μL of CCK8 (Dojindo, Japan) was added to each well, followed by a further incubation for 2 h. Finally, a multifunctional microplate reader (BioTek, Synergy2, Winooski, VT, USA) was employed to measure the OD value at 450 nm of each well.