Chromosomal localization and synteny analysis between J. curcas and the other three species (Arabidopsis, Populus, and rice) were performed using TBtools (program “Advanced circos” and “One StepMC ScanX”). Then we used the “Simple Ka/Ks Calculator (NG)” program of TBtools to calculate the rates of synonymous substitutions (Ks), nonsynonymous substitutions (Ka), and evolutionary rates (Ka/Ks ratio) of the J. curcas RR gene family. Scatter plot for the Ka/Ks value of duplicated JcRR gene pairs was constructed using R (https://www.r-project.org/, accessed on 11 October 2020, version 4.0.3).
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