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Enzyme-linked immunosorbent assay (ELISA) was performed to determine the levels of serum ApoE and ApoA1. The serum samples were centrifuged at 4 °C and 3500 rpm for 15 min; then, the supernatant was harvested and stored at −80 °C until use. ELISA was performed according to the protocol provided with the kit manual. Then, the absorbance was determined with a spectrophotometer (BioRad, CA, USA) at OD 450 nm and corresponding to standard curves.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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