4.11. Animals and Treatments

Weight-normalized mice were randomized into 6 groups (n = 6): (1) control group, (2) LPS group (5 mg/kg), (3) BGG + LPS group (20 mg/kg; 5 mg/kg), (4) BGG + LPS group (40 mg/kg; 5 mg/kg), (5) BGG group (20 mg/kg), (6) BGG group (40 mg/kg). BGG was administered to mice by the intra-peritoneal route. In the present study, after 1 h of BGG administration, a single systemic dose of LPS (5 mg/kg, i.p.) was administered to mice as systemic administration of LPS triggers acute inflammation. Afterward, mice were anesthetized by isoflurane and decapitated after 6 h of LPS treatment. Major organs like the liver, lungs, spleen, and kidneys were collected immediately and were stored in 10% phosphate-buffered formalin for histological analysis.