3.2. Induction of Fibromyalgia-like Condition: RIM6 and ASI Models

Two mouse models of fibromyalgia-like conditions were used in this study: reserpine-induced myalgia RIM6 mice [7,28] and intramuscular acid saline solution injection ASI mice [7,29]. Briefly, for the RIM6 model, reserpine (Sigma-Aldrich; St. Louis, MO, USA) dissolved in acetic acid and diluted to a final concentration of 0.5% acetic acid with saline solution was administered subcutaneously (0.25 mg/kg) on days 0, 1, 2, 9, 16, and 23. ASI mice were injected with acid saline solution (pH = 4, 10 µL) into the right gastrocnemius muscle, using a Hamilton syringe, on days 0 and 5. Following the same schedule of administrations, the corresponding control animals received either subcutaneous administration of reserpine dilution vehicle (CNT6 group) or intramuscular saline solution injection (CNT group).