4.8. Folding Transition Analysis Using Tycho NT.6

RepA-His₆ was analysed using Tycho NT.6 (NanoTemper Technologies GmbH, München, Germany) by applying a standard capillary (10 µL) with 0.5 mg/mL protein in His-tag buffer. Thermal unfolding profiles were recorded within a temperature gradient between 35 and 95 °C. In cases where the assays were performed with the addition of plasmid DNA, it was supplemented with 1 µg pWM321 10 min before start and incubated at room temperature or 37 °C.