VSV pseudotyped virus infection assays

NC Natalia Cheshenko
JB Jeffrey B. Bonanno
HH Hans-Heinrich Hoffmann
RJ Rohit K. Jangra
KC Kartik Chandran
CR Charles M. Rice
SA Steven C. Almo
BH Betsy C. Herold
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Infection of Vero or Huh7 cells by VSV-G, VSV-EBOV-GP, and VSV-S was monitored by plaque assay after 48 h culture and staining with crystal violet. Calu-3 cells were infected with virus and after 96 h culture, supernatants were harvested and viral yields quantified by titering on Vero cells.

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