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Data are presented as the mean ± standard error of mean (SEM). To detect significant changes among 3 or more groups, a one-way analysis of variance (ANOVA) was utilized and a post-hoc test (Tukey/LSD) was performed to account for multiple comparisons if the p value was < 0.05. The nonparametric Mann–Whitney test was used to detect significant changes among 2 groups. All statistical analyses were performed using GraphPad Prism (version: 9.0.0) software and statistical significance accepted at a two-sided p < 0.05.
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