2.3. Filtration, Cleaning, Centrifugation (Zeolite)

We filtered the infected water with 350 g of two types of zeolites, which were poured into the gauze stacked in the funnel of the filtration device. The zeolites had a particle thickness of 0.2–0.6 mm (Zeolite X) and 0–0.3 mm (Zeolite Y). We filtered 10 L of water with different concentrations of oocysts through each zeolite, washed with a solution containing 0.5% Tween 80 and 0.01% sodium polyphosphate. This was followed by centrifugation for 5 min by adding the sample to 5 mL centrifuge tubes at 5000 rpm. After draining the supernatant, we added distilled water to the test tubes and repeated the centrifugation procedure three times.