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2.6. Transmission Electron Microscopy (TEM)
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The lung tissue was minced into 1 mm3 cubes, fixed with 2.5% glutaraldehyde and 2% osmic acid, dehydrated and embedded in epoxy resin [21]. Ultrathin sections were collected onto 200-mesh copper grids, double stained with uranyl acetate and lead acetate, and then observed with a Hitachi H-7650 transmission electron microscope (Hitachi, Tokyo, Japan).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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