The monomers HPMA [28], 3-methacrylamidopropanoylthiazolidine-2-thione (Ma-AP-TT) [29] and cholest-5en-3β-yl 6-methacrylamido hexanoate (MA-AH-cholesterol) [30] were prepared according to the literature. High Performance Liquid Chromatography (HPLC) analysis was employed to verify the purity of monomers and chain transfer agents. Analysis was performed using a Shimadzu HPLC system with a C18 reversed-phase Chromolith Performance RP-18e column and a diode array detector (Shimadzu SPD-M20A), using water/acetonitrile (gradient of 5–95% v/v acetonitrile) as eluent with 5 mL min−1 flow rate.
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