Ultrasound examinations were performed on three groups of three mice each using a Vevo 2100 small animal ultrasound imaging system (Visualsonics, Toronto, Ontario, Canada). Animals’ chests were shaved the day before analysis. Mice were anesthetized by isoflurane inhalation, then fixed in the supine position on a 37 °C thermostatic fixation table, coated with a coupling agent, and the probe was placed on the left anterior thorax, and left ventricular motion was recorded in the short-axis view of the left ventricle using M-mode ultrasound.
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