Carrageenan-induced paw edema in ICR mice

To determine the anti-inflammatory effects in an animal model, carrageenan-induced paw edema was investigated [24, 25]. The animals were randomly divided into six groups (5 mice for each group). Group A received saline solution as control, Group B received STP at 10 mg/kg BW, Group C received RC at 100 mg/kg BW, Group D received LRC at 100 mg/kg BW, Group E-F received SRC at the dose of 50 and 100 mg/kg BW. All treatments were administered orally in mice. After 1 h of oral administration, the suspension of carrageenan at 0.5 mg/25 μL (Sigma-Aldrich, USA, Cat# C1867-1G) was injected into the subplantar tissue of the right hind paw, while the left hind paw was injected with saline solution. Paw edema was measured after carrageenan injection at 90, 180, 270, and 360 min to assess the difference in footpad thickness between the right and left foot.