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RNAi was performed using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150) with BLOCK-iT Fluorescent Oligo as a transfection control (Thermo Fisher Scientific, 13750062). Plxn knockdown was performed using Dharmacon ON-TARGETplus SMARTpool reagents with catalog numbers L-040789-01-0005, L-040790-01-0005, L-040791-01-0005, L-040980-00-0005, and L-056934-01-0005 for Plxna1, Plxna2, Plxna3, Plxnb2, and Plxnd1, respectively. Nrp oligos included Nrp1: GCACAAAUCUCUGAAACUA; and Nrp2: GACAAUGGCUGGACACCCA.
Copyright and License information: ©2022, Pinskey, Hoard et al
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