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2.8. Fourier transform infrared (FTIR) spectroscopy

KZ Kangyi Zhang
QW Qingyu Wen
YW Yufei Wang
TL Tianqi Li
BN Bin Nie
YZ Yu Zhang
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FTIR spectroscopy was detected using an infrared spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Samples of freeze‐dried powder and potassium bromide (KBr) were thoroughly mixed at a ratio of 1:150, ground into a fine powder, and compressed into thin slices. FTIR spectrum was scanned from 400 cm−1 to 4000 cm−1 wavelengths with 4 cm−1 resolution, and the number of scans was 64. Each sample was scanned in three replicate. Peak Fit software V4.12 was used to perform peak fitting and quantitative analysis of the secondary structure in the amide I region.

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