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Cells were plated in 12-well dishes (20,000 cells/well) and treated with different drugs for 48 h. The cells were subsequently stained with C11-BODIPY 581/591 (10 μM) for 30 min at 37 °C according to the manufacturer’s instruction. After washes in PBS, images were taken with a fluorescence microscope (ZEISS Axio Observer A1, Germany). The cells were harvested and analyzed via flow cytometry to detect oxidized forms of the probe. Each sample included at least 10,000 cells for analysis and all samples were tested in triplicate.
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