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Gene expression analysis

JS Jianwen Song
LS Lele Shang
CL Changxing Li
WW Wenqian Wang
XW Xin Wang
CZ Chunli Zhang
GA Guo Ai
JY Jie Ye
CY Changxian Yang
HL Hanxia Li
ZH Zonglie Hong
RL Robert M. Larkin
ZY Zhibiao Ye
JZ Junhong Zhang
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Pointed tips from the pointed tip accessions and the corresponding pericarp from the non-pointed tip accessions were collected from the distal end of the fruit and were immediately frozen in liquid nitrogen. Total RNA was extracted using TRIzol reagent (RN0102, Aidlab, China). First-strand cDNA was synthesized using HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper) (R212-01, Vazyme, China), as recommended by the manufacturer. Gene expression analysis was quantified using quantitative real-time PCR (qRT-PCR), which was conducted using QuantStudioTM 6 Flex System (ABI, USA). The tomato actin gene (Solyc11g008430) was used as an internal control. Primer sequences (designed using Primer Premier 5) are listed in Supplementary Data 7.

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