Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.10. N‐Acetylcysteine (NAC) supplementation in vivo
Request a Protocol
Ask a
question
Favorite
Eleven‐week‐old WT and Bco2 −/− female mice (n = 4–5/genotype) were treated with the antioxidant NAC (A1540922, ThermoFisher, Rockford, IL, USA) at a dose of 0.5 mg/g bodyweight in the drinking water for two weeks. Water was replaced every three days. Throughout the experiment, mice were maintained on a regular chow diet. At the end of the treatment, mice were euthanized between 9 a.m. and 11 a.m. and tissues were collected as described above.
Copyright and License information: ©2022 The Authors. published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
