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Circular dichroism (CD) spectroscopy
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The CD spectra were recorded for 40 μM peptide in a buffer consisting of 10 mM sodium phosphate, 30 mM sodium dodecyl sulfate (SDS), and 50% 2,2,2-trifluoroethanol (TFE). The CD spectra were acquired on a Jasco 810 spectropolarimeter (Jasco, Tokyo, Japan) equipped with a quartz cell with a 0.1-cm path length. The spectra were recorded at wavelengths from 190 nm to 250 nm.
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