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NAD+ levels were measured using a oxidized and reduced nicotinamide adenine dinucleotide (NAD/NADH) assay kit (ab65348; Abcam) according to the manufacturer's instructions. NAD+ and NADH levels were measured with a microplate reader spectrophotometer (Power Wave X‐1) at 450 nm. Values for NAD+ and NADH levels were determined from a standard calibration curve. NAD+ and NADH concentrations were normalized by protein content as measured using the Bradford assay.
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