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Determination by PCM

LJ Linlin Jiang
BZ Beibei Zhang
SL Shuitao Liu
LZ Lianqin Zhu
FZ Fenghua Zhu
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Then, 25 g (accurate to 0.0001 g) of ground feed was placed into a sterile flask, and 225 ml of phosphate buffer solution was added to the flask. Next, the solution was shaken for 5 min on a mechanical shaker. After standing for 5 min, the solution was made into a tenfold diluted sample homogenate, and a tenfold serial dilution was performed. The sample homogenate was diluted with accurate and quantitative sterile saline and allowed to stand for 10 min (in an environment of 2–4 °C), and then the supernatant was drawn for separation, culture and counting. The experiment was repeated 5 times for each sample. E. coli, Salmonella, S. aureus, LM, COLI, TVC, respectively, in accordance with the National Criterion of China GB/T 4789.38–2012 [25], GB/T 13,091–2018 [26], GB/T 4789.10–2016 [27], GB/T 4789.30–2016 [28], GB/T 18,869–2019 [29] and GB/T 13,093–2006 [30] were used for plate culture and counting.

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