2.4. Rotarod test

Motor performance and coordination were investigated by means of rotarod test in 16‐month‐old NURR1‐KO (male n = 6; female n = 9) and their WT littermates (male n = 6; female n = 5) and in 3–5‐month‐old female NURR1‐KO (n = 14) and their WT (n = 7) littermates. Mice were tested for three consecutive days. In each day, after a 2 min training session at a constant speed (4 rpm), the mice received three test sessions (T) in which the rod (Mouse Rotarod, Ugo Basile Biological Research Apparatus, Comerio, Italy) accelerated continuously from 4 to 65 rpm over 350 s (Hoxha et al., ²⁰¹³; Montarolo et al., ²⁰¹⁹). The latency to fall off the rod was recorded and reported as the mean of the third trials of each of the 3 days.