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2.1 K562 cells preparation and cell culture

CM Chandi T. Magawa
NE Natalie Eaton-Fitch
CB Cassandra Balinas
ES Etianne Martini Sasso
KT Kiran Thapaliya
LB Leighton Barnden
RM Rebekah Maksoud
BW Breanna Weigel
PR Penny A. Rudd
LH Lara J. Herrero
SM Sonya Marshall-Gradisnik
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The human erthryomyeloblastoid leukemia (K562) cell line was used to optimize protein extraction protocols prior to application in primary NK cells. K562 cells were cultured in Roswell Park Memorial Institute Medium (RPMI)-1640 (Invitrogen Life Technologies, Carlsbad, CA, United States) supplemented with 10% fetal bovine serum (FBS) (Invitrogen Life Technologies, Carlsbad, CA, United States) under physiological conditions in 5% CO2 at 37°C. Cultured K562 cells in exponential phase were prepared at 2.0 × 106 and 5.0 × 106 cells per sample in duplicates.

Copyright and License information:  ©2022 Magawa, Eaton-Fitch, Balinas, Sasso, Thapaliya, Barnden, Maksoud, Weigel, Rudd, Herrero and Marshall-Gradisnik.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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