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2.9 Intracellular iron measurement
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The content of intracellular ferrous iron (Fe2+) was measured using the Iron Assay kit (#ab83366, Abcam). Only ferrous (Fe2+) can bind to the iron probe to form a stable-colored complex that can be accessed by reading an absorption peak at a wavelength of 539 nm. Solutions of standard curve and reaction were prepared in accordance with the manufacturer’s instructions.
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