Differences between females and males and with IBS subtypes were analyzed using the χ 2 and Fisher's exact tests. Differences in the total scores on the IBS‐SSS, Birmingham IBS Symptom, FAS, IBS‐QoL, SF‐NDI, DI, and in fluorescence signals of fecal bacteria between responders and nonresponders were analyzed using the Mann–Whitney test. These analyses were performed using GraphPad Prism (version 8.0).
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