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In vitro intrinsic clearance calculation
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Measured values of parent chemical concentration were log10-transformed and plotted against time. A linear regression was performed on the substrate depletion data to estimate a first order elimination rate constant (k; equal to −2.3 × slope) with units of inverse time (1/h). In vitro intrinsic clearance values (CLin vitro, int, mL/h/106 cells) were then obtained by dividing k by 2 × 106 cells/mL, the nominal concentration of viable hepatocytes in the in vitro experiment (Fay et al., 2014b).
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