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Elevated plus maze

MB Megan L. Bertholomey
VN Vidhya Nagarajan
DS Dana M. Smith
MT Mary M. Torregrossa
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To measure anxiety-like behavior, rats were then tested in the elevated plus maze (EPM; MedPC; Pellow et al., 1985). The EPM consisted of two open and two closed arms (50 cm long, closed arms 42 cm high) raised 75 cm from the floor. Rats were placed in the center of the EPM under low illumination for 5 min, and number of entries (all four paws) into and time spent in the open and closed arms were measured. For all behavioral measures (EPM, FST, and fear conditioning), behavior was videorecorded and assessed by raters with no knowledge of the rats’ experimental condition.

Copyright and License information:  ©2022 Bertholomey, Nagarajan, Smith and Torregrossa.
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