Gene ontology (GO) analysis is a commonly applied method to define genes and its RNA or protein product to identify unique biological properties of high-throughput transcriptome or genome data. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis is a collection of datasets managing the genomes, diseases, biological pathways, drugs, and chemical materials. We utilize the Database for Annotation, Visualization, and Integrated Discovery (DAVID 6.8, https://david.ncifcrf.gov) to perform the GO analysis and KEGG analysis in helping classify the DEGs (P value < 0.05). The GO enrichment analysis comprises biological processes (BP), cellular components (CC), and molecular functions (MF).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.