All statistical analysis was performed in GraphPad Prism 7 software. Gene and protein expression data were log transformed prior to parametric tests. Student’s t-test or one-way ANOVA (with post-hoc tests) were used to test for significant differences between groups as indicated in the figure legend. If multiple genes or time points were tested in the same experiment, Bonferroni’s multiple correction testing was applied to account for the increased probability of type I errors. Linear regression analysis was performed to determine the association between plasma and brain imiquimod levels. A p-value of <0.05 was considered significant.
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