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Human serum was obtained through centrifuging healthy adult donors' peripheral blood at 500 × g for 5minutes at 4°C. Total IgG was purified from serum using affinity chromatography with Recombinant Protein G Agarose according to the manufacturer's instructions (Invitrogen, California, USA).
Copyright and License information: ©2016 Huang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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