RNA sample preparation and transcriptome analysis

AF Alireza Faridar
MV Matthew Vasquez
AT Aaron D. Thome
ZY Zheng Yin
HX Hui Xuan
JW Jing Hong Wang
SW Shixiang Wen
XL Xuping Li
JT Jason R. Thonhoff
WZ Weihua Zhao
HZ Hong Zhao
DB David R. Beers
SW Stephen T. C. Wong
JM Joseph C. Masdeu
SA Stanley H. Appel
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Using Trizol reagent, followed by Direct-zol RNA MiniPrep Kit (Zymo Research), messenger RNA was extracted from medial temporal cortex and frontal cortex of mice. For mouse neuroinflammation panel analysis, 770 transcripts were quantified with the NanoString nCounter multiplexed target platform (www.nanostring.com). nCounts of mRNA transcripts were normalized using the geometric means of 10 housekeeping genes (Csnk2a2, Ccdc127, Xpnpep1, Lars, Supt7l, Tada2b, Aars, Mto1, Tbp, and Fam104a).

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