Using Trizol reagent, followed by Direct-zol RNA MiniPrep Kit (Zymo Research), messenger RNA was extracted from medial temporal cortex and frontal cortex of mice. For mouse neuroinflammation panel analysis, 770 transcripts were quantified with the NanoString nCounter multiplexed target platform (www.nanostring.com). nCounts of mRNA transcripts were normalized using the geometric means of 10 housekeeping genes (Csnk2a2, Ccdc127, Xpnpep1, Lars, Supt7l, Tada2b, Aars, Mto1, Tbp, and Fam104a).
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