Expression Profiling of AhAPX Genes

The expression levels of all AhAPX genes at diverse developmental tissues/organs (embryo, cotyledon, testa, pericarp, peg, root and stem, root nodule, root tip, root, step tip, stem, leaf, and flower), under various hormones (ethylene, abscisic acid, paclobutrazol, brassinolide, and salicylic acid), and abiotic stress (drought and cold) conditions were evaluated using openly available transcriptome dataset of cultivated peanut (cultivar Shitouqi) at PGR database (see text footnote 1; BioProject PRJNA480120) (Zhuang et al., 2019). The detailed procedure for sample harvesting and data analysis is presented in our recent paper (Zhuang et al., 2019). Owing to the great differences in the expression trends, we normalize the log2 of fragments per kilobase of transcript per million (FPKM) values. Finally, the circular heat maps were designed by TBtools software.