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Flow cytometry analysis

FJ Feng Jiang
RY Rongfeng Yang
DX Diya Xue
RL Rong Li
MT Meiling Tan
ZZ Zhicong Zeng
LX Luhua Xu
LL Linling Liu
YS Yinzhi Song
FL Fengxia Lin
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Flow cytometry was used to detect the percentage of natural killer (NK) cells and dendritic cell (DC) subsets in the peripheral blood of mice. Peripheral blood was collected into tubes containing ethylenediaminetetraacetic acid. Peripheral blood mononuclear cells (PBMCs) were obtained from whole blood by density gradient centrifugation. PBMCs (1 × 106) were incubated with fluorescein isothiocyanate-conjugated rat anti-mouse antibodies against CD45/PE, NK1.1/APC, CD11C/PE-CY5, and CD3 for 60 min in the dark. The antibodies were purchased from Tonbo (Beijing, China). The cell samples were detected on Novocyte D2060R (Agilent, CA, USA), and the percentage of CD3–NK1.1+ NK cell subset and CD11C+NK1.1– DC subset was analyzed using NovoExpress 1.4.1.

Copyright and License information:  ©2022 Jiang, Yang, Xue, Li, Tan, Zeng, Xu, Liu, Song and Lin.
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