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Measurement of dsDNA and eosinophil granule protein

DL Dong-Hyun Lee
JJ Jae-Hyuk Jang
SS Soyoon Sim
YC Youngwoo Choi
HP Hae-Sim Park
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To measure eosinophil-derived DNA and eosinophil-derived neurotoxin (EDN), eosinophils were seeded on 48-well plates at a concentration of 5 × 105 cells/well. Cells were treated with 10 μg/mL epithelial antigen-specific antibodies for 18 hours. Eosinophils cultured in the medium without treatment served as background control. We harvested the supernatant and attached eosinophils, which were incubated with micrococcal nuclease at 37°C for 20 minutes. DNA levels were evaluated with Quant-iT™ PicoGreen dsDNA kit. Levels of human EDN were measured using ELISA kits (SKIMS-BIO Co., Seoul, Korea) according to the manufacturer’s recommendations.

Copyright and License information:  ©2022 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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