The newly generated 18S rRNA piroplasm sequences of bovines, horses, dogs, sheep, and goats ({"type":"entrez-nucleotide","attrs":{"text":"MZ573175","term_id":"2069317250"}}MZ573175, {"type":"entrez-nucleotide","attrs":{"text":"MZ573172","term_id":"2069317217"}}MZ573172, {"type":"entrez-nucleotide","attrs":{"text":"MZ573176","term_id":"2069317251"}}MZ573176, {"type":"entrez-nucleotide","attrs":{"text":"MZ573174","term_id":"2069317249"}}MZ573174, {"type":"entrez-nucleotide","attrs":{"text":"MZ573177","term_id":"2069317252"}}MZ573177, {"type":"entrez-nucleotide","attrs":{"text":"MZ573173","term_id":"2069317218"}}MZ573173, {"type":"entrez-nucleotide","attrs":{"text":"MZ573171","term_id":"2069317216"}}MZ573171, {"type":"entrez-nucleotide","attrs":{"text":"MZ701980","term_id":"2075977589"}}MZ701980, {"type":"entrez-nucleotide","attrs":{"text":"MZ573178","term_id":"2069317253"}}MZ573178) as well as available sequences in GenBank ({"type":"entrez-nucleotide","attrs":{"text":"MN629354","term_id":"1771280986"}}MN629354, {"type":"entrez-nucleotide","attrs":{"text":"MH257729","term_id":"1383769953"}}MH257729) were used to identify the unique restriction enzymes (RE) sites (GenScript Restriction Enzyme Map Analysis Tools, https://www.genscript.com/tools/restriction-enzyme-map-analysis). The group of parasites affecting an animal species was further analyzed to identify the unique RE enzymes for easy identification and differentiation.
Copyright and License information: The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences ©2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
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