Flow cytometry

Lung tissue dissociation was performed using Lung Dissociation Kit (Miltenyi Biotec). Next, cells were stimulated with Leukocyte Activation Cocktail with Golgi Plug (BD Pharmingen) for 3 hours. Extracellular and intracellular staining was performed according to the standard protocol using a panel of fluorochrome-labeled monoclonal antibodies ( Supplementary Materials – Supplementary Table S2 ). Cells were acquired using the FACSAria system (BD Biosciences) and analyzed with the FlowJo v.10 software (BD Biosciences). A representative gating strategy has been presented in the Supplementary Materials – Supplementary Figure S2A .