Preparation of CB Slices and Dispersed Glomus Cells.

Mice were killed and carotid bifurcations were removed and placed in cold PBS where CB were dissected. Mouse CB slices and dispersed CB glomus cells were prepared as described previously by our laboratory (53–55). Slices were maintained at 37 °C in a 5% CO₂ incubator for 24 to 48 h prior to use. Freshly dispersed cells were plated on glass coverslips treated with poly-l-lysine (Sigma) and maintained at 37 °C in a 5% CO₂ incubator for 24 h before use. Additional information on the preparation and culture of CB dispersed cells and slices is given in SI Appendix, Materials and Methods.