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Cell lines and viruses

RI Ravi F. Iyer
DE David M. Edwards
PK Philipp Kolb
HR Hans-Peter Raué
CN Chris A. Nelson
ME Megan L. Epperson
MS Mark K. Slifka
JN Jeffrey C. Nolz
HH Hartmut Hengel
DF Daved H. Fremont
KF Klaus Früh
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BSC 40 (ATCC; CRL2761), Chinese Hamster Ovary (CHO-K1) (ATCC; CCL61), and MC57G mouse fibroblast cells (ATCC; CRL2295) were maintained in DMEM (Corning) supplemented with 10% Fetal Bovine Serum (FBS) (Hyclone). A20 mouse B lymphoma cells (ATCC; TIB208) were maintained in RPMI 1640 (Corning) supplemented with 10% FBS. 293-F human embryonal kidney cells (Thermo Fisher Scientific; Cat.#: R79007) were maintained in 293 expression media (Thermo Fisher Scientific; Cat.#: 12338026).

VACV (Western Reserve) and CPXV (Brighton Red Strain; BR) recombinants were propagated in BSC40 cells maintained in MEM (Corning) supplemented with 5% FBS. Viruses were purified utilizing standard protocols, as we have previously reported [16].

BW5147 mouse thymoma cells (kindly provided by Ofer Mandelboim, Hadassah Hospital, Jerusalem, Israel) were maintained at 3x105 to 9x105 cells/ml in RPMI GlutaMAX (Gibco) supplemented with 10% (vol/vol) FCS, sodium pyruvate (1x, Gibco) and β-mercaptoethanol (0.1 mM, Sigma). Generation of BW5147 reporter cells stably expressing chimeric mouse FcγRs is described elsewhere [58].

Copyright and License information:  ©2022 Iyer et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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