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The harvested leaves and nodes were inoculated on MS containing 0.5, 1.0, 1.5, 2.0, and 3.0 mg/l of BAP (Sigma-Aldrich®) and 0.5 mg/l of 2,4-D (Sigma-Aldrich®) for induction of callus. Callus started to form within 10–14 days of inoculation and proliferated well with successive sub-culturing. The callus induction efficacy was noted after 4 weeks of inoculation. The proliferated shoots were sub-cultured every 3–4 weeks.
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This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
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