Bimolecular Fluorescence Complementation (BiFC) assays

PN Punita Nagpal
PR Paul H. Reeves
JW Jeh Haur Wong
LA Laia Armengot
KC Keun Chae
NR Nathaniel B. Rieveschl
BT Brendan Trinidad
VD Vala Davidsdottir
PJ Prateek Jain
WG William M. Gray
YJ Yvon Jaillais
JR Jason W. Reed
request Request a Protocol
ask Ask a question
Favorite

Entry clones of full length cDNA sequences without stop codons were used to recombine into the pSPYNE and pSPYCE destination vectors [76] using Gateway LR Clonase II Enzyme mix, to generate BiFC expression constructs. BiFC assays were performed as described previously [20].

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A