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Detection of cancer cell migration and invasion

YC Yimeng Cui
XW Xin Wang
LZ Lei Zhang
WL Wei Liu
JN Jinfeng Ning
RG Ruixue Gu
YC Yaowen Cui
LC Li Cai
YX Ying Xing
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Use the tip of a 10 μL pipette to form a scratch on a six-well plate covered with LUAD cells. By comparing the rate of wound healing and taking pictures under the microscope, the cell migration rate was finally counted. Transwell assay was conducted with the Corning Inc. transwell chamber. The invasion experiment required the participation of the matrigel matrix involved. Cell suspension arranged in the upper chamber contained 2x104 cells, besides, ingredient of the lower chamber was 600μL RPMI-1640 complemented with 10% FBS. The fixation of methanol and staining of 0.1% crystal violet for observing the migration and invasion efficiency after 24h or 48h, respectively. Fields were randomly selected in each membrane for capturing.

Copyright and License information:  ©2022 Cui, Wang, Zhang, Liu, Ning, Gu, Cui, Cai and Xing
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