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Sperm reactive oxygen species (ROS)
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Intracellular ROS of sperm was assessed with a ROS Assay Kit (SAS, Beijing, China) containing the 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) according to the manufacturer’s instruction. In brief, 1 µL H2DCFDA and 5 µL PI were added into the sperm suspension and mixed well, incubated at 37 °C for 30 min in the dark. the samples were centrifuged, suspended with PBS. Then, the samples were detected by flow cytometer.
Copyright and License information: ©2022 Gao et al.
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