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Lentivirus production and transduction
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A total of 2.6 μg psPAX2, 1.4 μg pMD2. G, and 4 μg pLenti-TRE-CasRx were co-transfected into 293T cells in a 6-cm dish. The medium was changed 24 h later. Culture suspension was collected 24 h later and filtered through a 0.45 μm filter. The suspension was then added to the cells in the presence of 8 μg/ml polybrene.
Copyright and License information: ©2022 Lv, Zhao, Ma, Zou, Li, Yan, Sun and Song.
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