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RNA FISH for NEAT1 on cell lines
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GC cells grown on coverslips were rinsed with phosphate-buffered saline (PBS) in DEPC and fixed with cold 4% paraformaldehyde for 5 min at RT. Subsequently, the cells were treated with Triton X-100 at a concentration of 0.3% for 30 min. NEAT1_1 probe was diluted by RNA FISH hybridization buffer (1:50) and incubated with cells overnight at 37 °C. Images were acquired using laser scanning confocal microscope ZEISS 510 (Germany).
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