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The infection assay was performed on PF-127 medium to evaluate the effect of the repulsive response induced by different biocontrol bacteria on infection of the plant roots (Dash et al., 2017). Using a pipette, ~300 J2s were added to 1.5 cm posterior to the root tips of tomato seedlings on 23% PF-127 medium in a Petri dish with a diameter of 3 cm. Approximately 5 μl bacterial culture was added near the root tips. Roots were stained by acid fuchsin at 48 h after inoculation, and the number of M. incognita J2s that invaded the host plant roots was counted.
Copyright and License information: ©2022 Zhao, Zhou, Zou, Zhang and Huang.
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