Thirteen pairs of human primary HCC and paired adjacent non-tumorous liver tissues were collected from the Wen Zhou Traditional Chinese Medicine Hospital. Human materials were obtained with informed consent, and the study was approved by the Wenzhou Hospital of Traditional Chinese Medicine Ethics Committee. Three paired HCC and non-tumorous liver tissues were used for high-throughput RNA se-quencing, and the other 10 paired HCC and non-tumorous liver tissues were used for QPCR detection of selected circRNAs.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.