Stool samples were collected by the participants using a stool collection kit (TechnoSuruga Laboratory, Co., Ltd., Shizuoka, Japan), suspended in guanidine thiocyanate (GTC) solution (100 mM Tris-HCL (pH 9.0), 40 mM Tris-EDTA (pH 8.0), 4 M guanidine thiocyanate, and 0.001% bromothymol blue and mailed at room temperature.
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