4.2. Animal Care

Fifty-seven-week-old male ICR mice (weighing 28–35 g) were purchased from Daehan Biolink (Eumsung, Korea). The mice were raised in a temperature, humidity, and ventilation-controlled environment at 23 °C, with a 12 h light/dark cycle. They consumed regular feed and sterilized distilled water for the one-week adaptation period. This study was approved by the Animal Care and Use Committee of Invivo company, Korea (IV-RB-02-2106-18) and was conducted according to the NIH Guide for the Care and Use of Laboratory Animals guidelines.