5.7. Yeast One-Hybrid Assay

The yeast one-hybrid assay was performed according to the manufacturer’s protocol in the “Matchmaker Gold Yeast One-Hybrid Library Screening System User Manual” (Clontech). Briefly, the pTATA-box-pAbAi vector was transferred into the yeast strain and grown on medium lacking uracil (Ura). We used different concentrations of aureobasidin A (AbA^r) to test the bait strain in a medium lacking Ura. The vectors of pGADT7:OsTBP2.1 were then transferred to the strains with pTATA-box-pAbAi, and the strains were grown on a medium lacking leucine with 800 ng mL⁻¹ AbA^r.