4.4. Cell Culture and Cell Viability Assay

The B16/F10 murine melanoma cells (ATCC^® CRL-6475) were obtained from the American Type Culture (ATCC, Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle medium (DMEM, St. Louis, Mo, USA) supplemented with 10% fetal bovine serum and 100 units/mL of penicillin/streptomycin at 37 °C in a humidified atmosphere containing 5% CO₂. Stock solutions (5 mg/mL) were prepared in dimethyl sulfoxide (DMSO, Sigma, St. Louis, MO, USA) and were then diluted to proper concentrations in the growth medium. Cell viability was evaluated by EZ-Cytox assay kit (Daeil Lab Service Co. Ltd., Seoul, Korea) as described previously [25].